Western blot analysis of Osteopontin in MOLT4 using a Osteopontin polyclonal antibody (Product # PA1-9076) at a concentration of 0.1 µg/mL. The cell lysate was prepared with RIPA buffer. The primary antibody was incubated for one hour and chemiluminescence was used for target detection.
Immunohistochemical analysis of Osteopontin in Human Placenta using a Osteopontin monoclonal antibody (Product #PA1-9076) at 5 µg/mL. The Human Placenta tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supplier Page from Thermo Fisher Scientific for Osteopontin Antibody